By Morris Kline
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Extra info for Solutions manual to Calculus: An intuitive and physical approach
58 They also demonstrated the method on a larger reaction scale using protonated solvents followed by drying the resin and reswelling in deuterated solvent for NMR analysis. An inert soluble aldehyde was added as an internal standard. On either reaction scale, the number of product equivalents y was calculated from its signal integral I2 in the following way: quantitation methods I2 È ˘ y = xÍ ˙, I + I 0 . 8) where x is the number of equivalents of soluble reagent yielding an integral I1. The integral I1 is normalized for the two protons of the dialdehyde.
The resonances of the resin-bound product used for quantitation must also be carefully selected. 8. QUANTITATION METHODS NMR quantitation of materials synthesized on solid-phase beads is challenging, since resin dynamics and susceptibility differences within the sample contribute to broad lines in the spectrum. Spectral resolution of the desired analyte resonances from those of the resin as well as the reference material is important for both precise and accurate quantitation of the combinatorial product.
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